Meet the Chiaro

 

Capturing forces in biology

 

Popular applications include:

  • Mechanobiology
  • Mechanotransduction
  • Developmental biology
  • Microstructures
  • Biophysics

 

Optimized for inverted microscopes

The Chiaro is compatible with almost any inverted microscope to enable (advanced) microscopy to be combined with force sensing simultaneously. For example consider using fluorescence microscopy combined with applying pico-Newton range forces to live cells.
 

Everything you need

Even the standard version of the Chiaro includes all the tools required to perform advanced force sensing experiments, including liquid compatible probes, automated find-surface approach, automated surface mapping and of course pN-range force sensing capabilities.

Chiaro technology

The Chiaro enables highly accurate and precise force experiments on any sample in any environment with high speed and great ease of use. These unique capabilities are enabled by making use of the Optics11 patented micro-machined fiber-optical sensors. The Chiaro can be used to gently bring a spherical glass tip in contact with a sample and record all generated force data simultaneously, both under static and dynamic operation.


 

Specifications

 

Young’s Modulus range 5 Pa up to 5 GPa
Probe stiffness range 0.01 N/m up to 10 kN/m
Probe tip shape Spherical or cylindrical
Probe tip size 5 µm up to 500 µm
Maximum displacement stroke 20 µm / 100 µm**
Min. force resolution 20 picoNewton
Sample stage travel 12 mm X 12 mm (X-Y)
Lateral mapping resolution > 1 µm
Mapping speed Up to 2 points / second
Motorized Z-travel 12 mm
Manual Z-stage travel 5 cm
Built-in camera magnification 10 X
Modes of operation Displacement / Load* / Indentation*
Dynamic analysis* 0.1 Hz* / 1 Hz* / 10 Hz*
In-software analysis Young’s Modulus (Hertz / O&P)
Data output format Tab separated text files, BMP

 
* Comes with Dynamic module
** Comes with extended Piezo module

Combine with microscopy

The Chiaro can be mounted to almost any new or existing inverted microscope. Using an individual mounting post, or direct mounting to an existing microscope table with mounting holes, the Chiaro is the ultimate companion for cell or microstructure research. Amongst the many combinations possible, often recurring combinations are brightfield, phase-contract, fluorescence or confocal microscopy.

Product characteristics

PROBE

The heart of the instrument: a micromechanical all-optical indentation probe

MOUNT

The Chiaro can be combined with almost all inverted microscopes

XYZ COARSE STAGE

Map your samples using the 12 · 12 · 12 mm X-Y-Z movement range

FINE INDENTATION STAGE

A coarse-fine stage allows accurate indentation and automatic surface finding

RETRACTABLE ARM

To create room for samples and sample containers of any dimension

CHIARO HEAD

Designed for high flexibility and performance

Created with Sketch.
Ernst Breel

Interested to learn more about our Chiaro instrument?

Feel free to contact Ernst to explore the possibilities.

ernst.breel [at] optics11.com +31 20 598 8326